DynAMic research plan comprises of six Work Packages (WPs) acting synergistically and complimentarily towards the goals of the project, with clearly defined objectives and milestones.
WP1: Project management (CO: FORTH) runs horizontally throughout the project and is related to the coordination, management, decision making, planning of activities and adjusting the workflow between the partners. WP1 focuses also on the management of the project’s resources related to the human, financial and technological means of the consortium. It involves the following tasks: 1) supervision of the project’s vision and strategy, 2) management of resources, 3) decision making and implementation, 4) coordination of procedures and communication, 5) execution of administrative actions both internal and with the Commission, 6) supervision of external activities and 7) supervision of gender equality.
WP2: Adaptive Optronics (CO: USTAN) focuses on the next generation optomechatronic enabling the ultrafast manipulation and detection of light propagating through living tissue with novel adaptive wavefront control technologies for non-diffracting speckle illumination, appropriate spatial filtering and innovative guiding-star mechanisms. Our photonics and optoelectronics industrial partners Imagine Optic and Rayfos, will contribute to the design, optimization and utilization of the ultrafast adaptive optomechatronic components and advanced data acquisition with scalable processing elements (FPGA) and a sophisticated control module (GPU).
WP3: Algorithms (CO: CNRS) focuses on developing and optimizing our ultrafast, real time theoretical methods and advanced algorithms enabling the extraction and quantitative measurement of hidden features and arbitrary shapes and images through opaque media, regardless of the amount of disorder. These approaches will exploit our knowhow on adaptive modelling of light transport in complex media, and 3D autocorrelation phase and polarization retrieval in optical and optoacoustic imaging and mesoscopic transmission matrix calculation algorithms.
WP4: System Integration (CO: TUM) is dedicated to the realization of the complete next generation microscopy system with all developed technologies and algorithms implemented and utilized. The complete microscopy system will incorporate all modalities in a modular concept providing unique capabilities for elucidating tissue components and biomolecules at different scales and resolutions as well as permitting the potential end user the selection of technology, application and cost. A schematic representation of our modular, adaptive microscopy concept is shown in the Figure, with all developed technologies integrated in a conventional system, imaging a scattering sample through an opaque lens and controlled by a complete set of advanced algorithms.
WP5: System Implementation (CO: EKUT) and with the participation of all partners is dedicated to the system implementation in our specific and targeted biological and translational applications. Proof of concept will be based on tissue samples including retinal explants from surgically enucleated eyes (as a consequence of malignant tumors) while our clinical application will target the ocular cavity with the retina and the optic nerve providing access to the brain and related pathologies and neurologic disorders. In vivo studies will be performed to demonstrate the superiority of the new microscopy system and link molecular fingerprints to high content proteomic peptide mass-fingerprints.
WP6: Dissemination and exploitation (CO: Rayfos and Imagine Optic) is focused on dissemination to the scientific community and to general public, exploitation and IPR protection. Dissemination and communication activities include publishing of the project’s results to high-ranked peer-reviewed international journals and conferences and to the general public via e.g. visits to fairs and public bodies (i.e. schools), exhibitions, newspapers, journals, web-pages or TV/radio reports. WP6 includes also the Data Management Plan and the design of our exploitation plan and IPR management.
M1 Consortium Agreement (WP1, M6): Consortium agreement completed and signed defining the operational guidelines for the project
M2 Smart illumination (WP2, M12): Smart illumination strategies ready for testing
M3 Adaptive control (WP2, M18): Adaptive control strategies ready for testing
M4 Initial algorithms (WP3, M12): Adaptive optics algorithms ready for evaluation
M5 Phase retrieval (WP3, M20): Initial functional phase retrieval algorithms
M6 SRS integration (WP4, M18): First integrated prototype with SRS capability
M7 Adaptive module (WP4, M24): First prototype of the complete set of adaptive optics technologies and methodologies
M8 Initial evaluation (WP5, M24): Initial evaluation of the integrated system
M9 First in vivo tests (WP5, M36): Initial in vivo animal experimental tests
M10 Exploitation roadmap (WP6, M18): Report on the exploitation roadmap produced and evaluated by the partners